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rarg crispr cloning vector plenticrispr v2  (GenScript corporation)

 
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    Structured Review

    GenScript corporation rarg crispr cloning vector plenticrispr v2
    mRNA expression levels of RARs in cholangiocarcinoma cells and effect of ATRA on cell viability. (A) KKU-100 and (B) KKU-213B cells were treated with increasing concentrations of ATRA for 12, 24, and 48 h. SRB staining was performed to detect cell viability. Data are presented as the mean ± SD from three independent experiments. (C) mRNA expression levels of RARA, RARB and <t>RARG</t> in KKU-100 and KKU-213B cells were quantified using RT-qPCR and normalized to ACTB . Data from two independent experiments are presented. *P<0.05, **P<0.01 and ***P<0.001 vs. untreated control. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor.
    Rarg Crispr Cloning Vector Plenticrispr V2, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rarg crispr cloning vector plenticrispr v2/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    rarg crispr cloning vector plenticrispr v2 - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "All- trans -retinoic acid induces RARB-dependent apoptosis via ROS induction and enhances cisplatin sensitivity by NRF2 downregulation in cholangiocarcinoma cells"

    Article Title: All- trans -retinoic acid induces RARB-dependent apoptosis via ROS induction and enhances cisplatin sensitivity by NRF2 downregulation in cholangiocarcinoma cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2022.13299

    mRNA expression levels of RARs in cholangiocarcinoma cells and effect of ATRA on cell viability. (A) KKU-100 and (B) KKU-213B cells were treated with increasing concentrations of ATRA for 12, 24, and 48 h. SRB staining was performed to detect cell viability. Data are presented as the mean ± SD from three independent experiments. (C) mRNA expression levels of RARA, RARB and RARG in KKU-100 and KKU-213B cells were quantified using RT-qPCR and normalized to ACTB . Data from two independent experiments are presented. *P<0.05, **P<0.01 and ***P<0.001 vs. untreated control. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor.
    Figure Legend Snippet: mRNA expression levels of RARs in cholangiocarcinoma cells and effect of ATRA on cell viability. (A) KKU-100 and (B) KKU-213B cells were treated with increasing concentrations of ATRA for 12, 24, and 48 h. SRB staining was performed to detect cell viability. Data are presented as the mean ± SD from three independent experiments. (C) mRNA expression levels of RARA, RARB and RARG in KKU-100 and KKU-213B cells were quantified using RT-qPCR and normalized to ACTB . Data from two independent experiments are presented. *P<0.05, **P<0.01 and ***P<0.001 vs. untreated control. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor.

    Techniques Used: Expressing, Staining, Quantitative RT-PCR, Control

    ATRA cytotoxicity in cholangiocarcinoma cells is partly RAR-dependent. KKU-213B cells were transfected with siRARA, siRARB, siNT or RARG CRISPR. The RAR expression and cell viability of cells were transfected with (A and B) siRARA, (C and D) siRARB and (E and F) RARG CRISPR are shown. Protein expression level was assessed by western blot analysis and cell viability was measured following treatment with increasing concentrations of ATRA for 48 h. Data are presented as the mean ± SD from two independent experiments. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor; NT, non-targeting; si, small interfering.
    Figure Legend Snippet: ATRA cytotoxicity in cholangiocarcinoma cells is partly RAR-dependent. KKU-213B cells were transfected with siRARA, siRARB, siNT or RARG CRISPR. The RAR expression and cell viability of cells were transfected with (A and B) siRARA, (C and D) siRARB and (E and F) RARG CRISPR are shown. Protein expression level was assessed by western blot analysis and cell viability was measured following treatment with increasing concentrations of ATRA for 48 h. Data are presented as the mean ± SD from two independent experiments. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor; NT, non-targeting; si, small interfering.

    Techniques Used: Transfection, CRISPR, Expressing, Western Blot

    IC 50 values of ATRA in KKU-213B cells following RAR knockdown/knockout.
    Figure Legend Snippet: IC 50 values of ATRA in KKU-213B cells following RAR knockdown/knockout.

    Techniques Used: Knockdown, CRISPR



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    rarg crispr cloning vector plenticrispr v2  (GenScript corporation)
    90
    GenScript corporation rarg crispr cloning vector plenticrispr v2
    mRNA expression levels of RARs in cholangiocarcinoma cells and effect of ATRA on cell viability. (A) KKU-100 and (B) KKU-213B cells were treated with increasing concentrations of ATRA for 12, 24, and 48 h. SRB staining was performed to detect cell viability. Data are presented as the mean ± SD from three independent experiments. (C) mRNA expression levels of RARA, RARB and <t>RARG</t> in KKU-100 and KKU-213B cells were quantified using RT-qPCR and normalized to ACTB . Data from two independent experiments are presented. *P<0.05, **P<0.01 and ***P<0.001 vs. untreated control. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor.
    Rarg Crispr Cloning Vector Plenticrispr V2, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rarg crispr cloning vector plenticrispr v2/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    rarg crispr cloning vector plenticrispr v2 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    mRNA expression levels of RARs in cholangiocarcinoma cells and effect of ATRA on cell viability. (A) KKU-100 and (B) KKU-213B cells were treated with increasing concentrations of ATRA for 12, 24, and 48 h. SRB staining was performed to detect cell viability. Data are presented as the mean ± SD from three independent experiments. (C) mRNA expression levels of RARA, RARB and RARG in KKU-100 and KKU-213B cells were quantified using RT-qPCR and normalized to ACTB . Data from two independent experiments are presented. *P<0.05, **P<0.01 and ***P<0.001 vs. untreated control. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor.

    Journal: Oncology Letters

    Article Title: All- trans -retinoic acid induces RARB-dependent apoptosis via ROS induction and enhances cisplatin sensitivity by NRF2 downregulation in cholangiocarcinoma cells

    doi: 10.3892/ol.2022.13299

    Figure Lengend Snippet: mRNA expression levels of RARs in cholangiocarcinoma cells and effect of ATRA on cell viability. (A) KKU-100 and (B) KKU-213B cells were treated with increasing concentrations of ATRA for 12, 24, and 48 h. SRB staining was performed to detect cell viability. Data are presented as the mean ± SD from three independent experiments. (C) mRNA expression levels of RARA, RARB and RARG in KKU-100 and KKU-213B cells were quantified using RT-qPCR and normalized to ACTB . Data from two independent experiments are presented. *P<0.05, **P<0.01 and ***P<0.001 vs. untreated control. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor.

    Article Snippet: The RARG CRISPR cloning vector pLentiCRISPR v2 was purchased from GenScript ® (RARG CRISPR guide RNA 2; Cat. no. SC1805), sequence of the gRNA is shown in .

    Techniques: Expressing, Staining, Quantitative RT-PCR, Control

    ATRA cytotoxicity in cholangiocarcinoma cells is partly RAR-dependent. KKU-213B cells were transfected with siRARA, siRARB, siNT or RARG CRISPR. The RAR expression and cell viability of cells were transfected with (A and B) siRARA, (C and D) siRARB and (E and F) RARG CRISPR are shown. Protein expression level was assessed by western blot analysis and cell viability was measured following treatment with increasing concentrations of ATRA for 48 h. Data are presented as the mean ± SD from two independent experiments. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor; NT, non-targeting; si, small interfering.

    Journal: Oncology Letters

    Article Title: All- trans -retinoic acid induces RARB-dependent apoptosis via ROS induction and enhances cisplatin sensitivity by NRF2 downregulation in cholangiocarcinoma cells

    doi: 10.3892/ol.2022.13299

    Figure Lengend Snippet: ATRA cytotoxicity in cholangiocarcinoma cells is partly RAR-dependent. KKU-213B cells were transfected with siRARA, siRARB, siNT or RARG CRISPR. The RAR expression and cell viability of cells were transfected with (A and B) siRARA, (C and D) siRARB and (E and F) RARG CRISPR are shown. Protein expression level was assessed by western blot analysis and cell viability was measured following treatment with increasing concentrations of ATRA for 48 h. Data are presented as the mean ± SD from two independent experiments. ATRA, all- trans -retinoic acid; RAR, retinoic acid receptor; NT, non-targeting; si, small interfering.

    Article Snippet: The RARG CRISPR cloning vector pLentiCRISPR v2 was purchased from GenScript ® (RARG CRISPR guide RNA 2; Cat. no. SC1805), sequence of the gRNA is shown in .

    Techniques: Transfection, CRISPR, Expressing, Western Blot

    IC 50 values of ATRA in KKU-213B cells following RAR knockdown/knockout.

    Journal: Oncology Letters

    Article Title: All- trans -retinoic acid induces RARB-dependent apoptosis via ROS induction and enhances cisplatin sensitivity by NRF2 downregulation in cholangiocarcinoma cells

    doi: 10.3892/ol.2022.13299

    Figure Lengend Snippet: IC 50 values of ATRA in KKU-213B cells following RAR knockdown/knockout.

    Article Snippet: The RARG CRISPR cloning vector pLentiCRISPR v2 was purchased from GenScript ® (RARG CRISPR guide RNA 2; Cat. no. SC1805), sequence of the gRNA is shown in .

    Techniques: Knockdown, CRISPR